GMO detection and identification

The hardware&methodology complex, offered by GenBit LLC, provides for one-step simultaneous detection and identification of multiple genetically modified organism of plant origin according to the testing algorithm given by Methodical Guidelines of the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor) from 03.08.2016г. and by Methodical Recommendations of the Ministry of agriculture of the Russian Federation (enforced by the order of the Minister No 2 from 10.01.2017г.).
Matrix method proposed and used by GenBit LLC is fully complementary to the matrix approach to the detection and identification of GMOs used in the European Union, while being faster, more flexible, posing significantly less rigid requirements for the personnel qualification.
The method employs two-dimensional open-type matrices each node (microreactor) of which (30 or 48) contains stabilised PCR mixture necessary to determine specific object (specific nucleotide sequence - transformation event, genetic element…). The mixtures include primers, probes, hot start taq-polymerase and dNTPs required for PCR.
Set of test systems, used by GenBit, makes it possible to configure matrices enabling detection and identification of more than 90% of all transformation events authorized worldwide. Matrix configuration can be freely varied both for screening "plain" samples (single crop, e.g., "GMO: maize" , "GMO: soybean ), and samples of unknown composition (e.g., "GMO: Screening", "GMO: Markers").
Special technology of immobilization and stabilization of PCR mixtures on the surface of microreactors ensures long shelf life of matrices for 6 months at room temperature with no special conditions, and reduces the number of necessary manipulations (it is only necessary to apply DNA sample on the matrix). The analysis can be performed in both stationary and field laboratories. small volume of reaction (1.2 μl) reduces consumption of reagents and total duration of the assay to 1.5 hours, including DNA extraction stage.
Genetically modified crops database, supported by GenBit, provides for definition of transformation event when no specific lines have been found, but elements of genetically engineered constructs are identified in the sample. Other reliable information resources can be used for the same purpose.