Genbit: selected publications
2021, "Russian Federation. Federal Service for Intellectual Property. Bul. № 14.". The invention relates to the field of biotechnology, namely to molecular diagnostics, and can be used in the food industry for matrix identification of fish species of the Gadidae (cod) family in food products and food raw materials. The proposed method makes it possible to identify all species of cod fish within the framework of one analysis and obtain the analysis result faster than existing methods.
2019, "American Journal of Biometrics and Biostatistics". An approach to the exact quantitative analysis of seed traits without using reference material is proposed basing on the quantum (discreet) nature of seed distribution is seed lots. The approach has been proven theoretically and supported experimentally with the analysis of the presence of seeds of soybean lines A2704-12, A5547-127 and GTS 40-3-2 in seed lots with the use of PCR in real-time mode tracing the transformation events
2019, "Pathogens".The study is the first large-scale screening of a wide range of potato pathogens conducted in network mode using unified methodology and standardized qPCR micromatrices. The data represent valuable information for plant pathologists and potato producers and indicate the high potential of the combined use of matrix PCR technology and network approaches to data collection and analysis with the view to rapidly and accurately assess the prevalence of certain pathogens, as well as the phytosanitary state of large territories
2019, AIP Conference Proceedings. In this study we developed test system for real-time PCR detection and identification of Synchytrium endobioticum suitable for use in qPCR micromatrices providing for multiplex detection of a range of pathogens. The designed primers and probe were successfully tested for specificity on 10 samples of non-target fungal potato pathogens. Detection limit was 0.1 pg of DNA per reaction. Reproducibility assay showed the standard error for Ct value did not exceed 4.5%
2018, "Biosensors". In this study, diagnostic systems have been developed for simultaneous detection and identification of six fungal pathogens using 48-well microarrays (micromatrices) for qPCR. All oligonucleotide sets were tested for their specificity using 59 strains of target and non-target species. Detection limit of the developed test systems varied from 0.6 to 43.5 pg of DNA depending on target species with reproducibility within 0.3−0.7% (standard deviation)
2018, "Journal of Applied Microbiology". The method for highly sensitive, selective, rapid and easy‐to‐use detection and identification of economically significant potato pathogens, including viruses, bacteria and oomycetes, be it single pathogen, or a range of various pathogens that might occur simultaneously. The accurate, rapid and user‐friendly diagnostic system in a micromatrix format may significantly contribute to pathogen screening and phytopathological studies
2018, "Russian Journal of Nematology". Based on ITS1-5.8S-ITS2-28S rDNA sequences, test systems for detection of Ditylenchus destructor and D. dipsaci, and also for D. weischeri, a confounding species, were developed suitable for the use in complex qPCR micromatrices (microarrays) under standardised real-time PCR protocol.
2018, "Protection and Quarantine of Plants". New test-system developed for detection and identification of Spongospora subterranea with real-time PCR along with assessment of it's sensitivity, selectivity and the potential practical use of the developed approach
2018, "Modern approaches and methods in plant protection", A new approach to the diagnosis of pathogenic microorganisms was applied, based on the use of qPCR microarrays (microarrays), which provides for simultaneously conducting 30-48 independent amplification reactions on one microchip using standard protocol on AriaDNA microchip amplifier
2017, "Russian Journal of Nematology". The easy, rapid and inexpensive system for the complex real-time multiplex PCR diagnostics of two potato cyst nematode species, Globodera rostochiensis and Globodera pallida, using disposable long-stored stationary PCR microarrays. Laboratory trials have demonstrated an excellent diagnostic efficiency and sensitivity of the developed test systems with the DNA detection limit equal to 1 and 10 pg for G. rostochiensis and G. pallida, respectively
2017, "Potato and Vegtables" Real-time PCR diagnostics using disposable PCR matrices is a promising method to screen samples for a wide range of pathogens. In 2015-2017 a massive screening based on PCR matrices was done for detection of eight viral/viroid potato pathogens - PVY (including ordinary (PVYO) and necrotic (PVYNTN) forms), PVX, PVM, PVS, PVA, PLRV, PMTV, and PSTVd - in 11 regions of Russia in real time
2016, "Biotechnologia Acta", Test-systems designed on the base of Desulfotomaculum, Desulfovibrio and Desulfobulbus genera sequences with their use in matrix real-time PCR provided for faster and more efficient identification of corrosive-relevant sulfate-reducing bacteria that conventional approaches do. This approach will serve as a base for monitoring of these bacteria for estimating corrosion sites
2015, "Biotechnology in Russia", It has been shown by PCR with various primers that the commercial “Raudis” Spring rapeseed breed presented as non-GMO naturally tolerant to glyphosate, and obtained by conventional breeding is indeed a genetically modified organism that contains genetic construct typical of GT73 line (Monsanto). This construct is most likely responsible for experimentally confirmed glyphosate tolerance of the breed
2015, "Protection and Quarantine of Plants". Result are given for ~2.5K tests of potato plants in several regions of Russia obtained through in-field testing with the use of Lateral Flow Strip technique